Isolation and Characterization of Cellulose Producing Fungi from Soil in Abraka, Delta State, Nigeria

Adomi Patience Ogheneochuko; Anozie Anthony Jude

doi:doi:10.11648/j.ijmb.20230803.12

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Isolation and Characterization of Cellulose Producing Fungi from Soil in Abraka, Delta State, Nigeria

Adomi Patience Ogheneochuko, Anozie Anthony Jude

Published in International Journal of Microbiology and Biotechnology (Volume 8, Issue 3)

Received: 1 March 2023 Accepted: 24 March 2023 Published: 31 August 2023

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Abstract

Cellulase is an important enzyme which is essential for industrial production of paper, animal feeds and other products useful for humans. Previous study showed that bacteria and fungi from different sources produce cellulase. Few reports exist on the production of cellulase from soil fungi. The purpose of the study was to isolate fungi from soil and screen fungi for cellulase production. Soil samples collected from different sites were serially diluted and plated out into standard mycologic media. Pure isolates were screened for cellulase production using carboxylmethyl cellulase agar. Effects of temperature, incubation time, carbon source, nitrogen source, agricultural wastes and other factors were determined for cellulase production. Eighteen fungal isolates were isolated. Fourteen hydrolysed the medium for cellulase production however isolates 1FG and 8FG that produced higher zones of inhibition were selected for further study. Morphology, lactophenol staining and molecular characterization indicated these isolates as Aspergillus sp and Fusarium sp. Arabinose and yam peels showed better results among the other carbon sources. Peptone and temperatures at 45°C and 55°C respectively were best nitrogen source and conditions for enzyme production. This investigation showed that fungi isolated from soil have potential for cellulase production, which could be used for large scale production of the enzyme.

Published in	International Journal of Microbiology and Biotechnology (Volume 8, Issue 3)
DOI	10.11648/j.ijmb.20230803.12
Page(s)	54-61
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2023. Published by Science Publishing Group

Keywords

Cellulase Producing Fungi, Arabinose, Peptone

References

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APA Style

Adomi Patience Ogheneochuko, Anozie Anthony Jude. (2023). Isolation and Characterization of Cellulose Producing Fungi from Soil in Abraka, Delta State, Nigeria. International Journal of Microbiology and Biotechnology, 8(3), 54-61. https://doi.org/10.11648/j.ijmb.20230803.12

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ACS Style

Adomi Patience Ogheneochuko; Anozie Anthony Jude. Isolation and Characterization of Cellulose Producing Fungi from Soil in Abraka, Delta State, Nigeria. Int. J. Microbiol. Biotechnol. 2023, 8(3), 54-61. doi: 10.11648/j.ijmb.20230803.12

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AMA Style

Adomi Patience Ogheneochuko, Anozie Anthony Jude. Isolation and Characterization of Cellulose Producing Fungi from Soil in Abraka, Delta State, Nigeria. Int J Microbiol Biotechnol. 2023;8(3):54-61. doi: 10.11648/j.ijmb.20230803.12

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@article{10.11648/j.ijmb.20230803.12,
  author = {Adomi Patience Ogheneochuko and Anozie Anthony Jude},
  title = {Isolation and Characterization of Cellulose Producing Fungi from Soil in Abraka, Delta State, Nigeria},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {8},
  number = {3},
  pages = {54-61},
  doi = {10.11648/j.ijmb.20230803.12},
  url = {https://doi.org/10.11648/j.ijmb.20230803.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20230803.12},
  abstract = {Cellulase is an important enzyme which is essential for industrial production of paper, animal feeds and other products useful for humans. Previous study showed that bacteria and fungi from different sources produce cellulase. Few reports exist on the production of cellulase from soil fungi. The purpose of the study was to isolate fungi from soil and screen fungi for cellulase production. Soil samples collected from different sites were serially diluted and plated out into standard mycologic media. Pure isolates were screened for cellulase production using carboxylmethyl cellulase agar. Effects of temperature, incubation time, carbon source, nitrogen source, agricultural wastes and other factors were determined for cellulase production. Eighteen fungal isolates were isolated. Fourteen hydrolysed the medium for cellulase production however isolates 1FG and 8FG that produced higher zones of inhibition were selected for further study. Morphology, lactophenol staining and molecular characterization indicated these isolates as Aspergillus sp and Fusarium sp. Arabinose and yam peels showed better results among the other carbon sources. Peptone and temperatures at 45°C and 55°C respectively were best nitrogen source and conditions for enzyme production. This investigation showed that fungi isolated from soil have potential for cellulase production, which could be used for large scale production of the enzyme.},
 year = {2023}
}

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TY - JOUR
T1 - Isolation and Characterization of Cellulose Producing Fungi from Soil in Abraka, Delta State, Nigeria
AU - Adomi Patience Ogheneochuko
AU - Anozie Anthony Jude
Y1 - 2023/08/31
PY - 2023
N1 - https://doi.org/10.11648/j.ijmb.20230803.12
DO - 10.11648/j.ijmb.20230803.12
T2 - International Journal of Microbiology and Biotechnology
JF - International Journal of Microbiology and Biotechnology
JO - International Journal of Microbiology and Biotechnology
SP - 54
EP - 61
PB - Science Publishing Group
SN - 2578-9686
UR - https://doi.org/10.11648/j.ijmb.20230803.12
AB - Cellulase is an important enzyme which is essential for industrial production of paper, animal feeds and other products useful for humans. Previous study showed that bacteria and fungi from different sources produce cellulase. Few reports exist on the production of cellulase from soil fungi. The purpose of the study was to isolate fungi from soil and screen fungi for cellulase production. Soil samples collected from different sites were serially diluted and plated out into standard mycologic media. Pure isolates were screened for cellulase production using carboxylmethyl cellulase agar. Effects of temperature, incubation time, carbon source, nitrogen source, agricultural wastes and other factors were determined for cellulase production. Eighteen fungal isolates were isolated. Fourteen hydrolysed the medium for cellulase production however isolates 1FG and 8FG that produced higher zones of inhibition were selected for further study. Morphology, lactophenol staining and molecular characterization indicated these isolates as Aspergillus sp and Fusarium sp. Arabinose and yam peels showed better results among the other carbon sources. Peptone and temperatures at 45°C and 55°C respectively were best nitrogen source and conditions for enzyme production. This investigation showed that fungi isolated from soil have potential for cellulase production, which could be used for large scale production of the enzyme.
VL - 8
IS - 3
ER -

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Author Information

Adomi Patience Ogheneochuko

Department of Microbiology, Faculty of Science, Delta State University, Abraka, Nigeria
Anozie Anthony Jude

Mediaan Medical Laboratory, Abraka, Nigeria

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APA Style

Adomi Patience Ogheneochuko, Anozie Anthony Jude. (2023). Isolation and Characterization of Cellulose Producing Fungi from Soil in Abraka, Delta State, Nigeria. International Journal of Microbiology and Biotechnology, 8(3), 54-61. https://doi.org/10.11648/j.ijmb.20230803.12

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ACS Style

Adomi Patience Ogheneochuko; Anozie Anthony Jude. Isolation and Characterization of Cellulose Producing Fungi from Soil in Abraka, Delta State, Nigeria. Int. J. Microbiol. Biotechnol. 2023, 8(3), 54-61. doi: 10.11648/j.ijmb.20230803.12

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AMA Style

Adomi Patience Ogheneochuko, Anozie Anthony Jude. Isolation and Characterization of Cellulose Producing Fungi from Soil in Abraka, Delta State, Nigeria. Int J Microbiol Biotechnol. 2023;8(3):54-61. doi: 10.11648/j.ijmb.20230803.12

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@article{10.11648/j.ijmb.20230803.12,
  author = {Adomi Patience Ogheneochuko and Anozie Anthony Jude},
  title = {Isolation and Characterization of Cellulose Producing Fungi from Soil in Abraka, Delta State, Nigeria},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {8},
  number = {3},
  pages = {54-61},
  doi = {10.11648/j.ijmb.20230803.12},
  url = {https://doi.org/10.11648/j.ijmb.20230803.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20230803.12},
  abstract = {Cellulase is an important enzyme which is essential for industrial production of paper, animal feeds and other products useful for humans. Previous study showed that bacteria and fungi from different sources produce cellulase. Few reports exist on the production of cellulase from soil fungi. The purpose of the study was to isolate fungi from soil and screen fungi for cellulase production. Soil samples collected from different sites were serially diluted and plated out into standard mycologic media. Pure isolates were screened for cellulase production using carboxylmethyl cellulase agar. Effects of temperature, incubation time, carbon source, nitrogen source, agricultural wastes and other factors were determined for cellulase production. Eighteen fungal isolates were isolated. Fourteen hydrolysed the medium for cellulase production however isolates 1FG and 8FG that produced higher zones of inhibition were selected for further study. Morphology, lactophenol staining and molecular characterization indicated these isolates as Aspergillus sp and Fusarium sp. Arabinose and yam peels showed better results among the other carbon sources. Peptone and temperatures at 45°C and 55°C respectively were best nitrogen source and conditions for enzyme production. This investigation showed that fungi isolated from soil have potential for cellulase production, which could be used for large scale production of the enzyme.},
 year = {2023}
}

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