Marek's disease virus (MDV) is a highly cell-associated oncogenic alphaherpesvirus that causes chicken T-cell lymphoma. MD is currently controlled by vaccination; however, MDV strains tend to develop increased virulence. Distinct diversity and point mutations are present in the Meq proteins, the major oncoproteins of MDV, suggesting that changes in protein function induced by amino acid substitutions might affect MDV virulence. This study sampled 30 commercial broiler flocks from different Provinces (10 spleens from each flock) at slaughter. Gallid alphaherpesvirus 2 was identified in PCR (using gb primer) of spleen samples of 11 flocks (36.67%). Two provinces, Azerbaijan Gharbi (44%) and Golestan (25%), recorded the highest and lowest infection rates. The oncogene Meq of some positive samples was amplified by PCR and sequenced. MDV strains detected in this study could be put in three branches, with molecular features consistent with virulent and very virulent previously identified MDV. UT-PCR9303 was located with an Iraqi isolate. UT-PCR-9231 had high homology with an Iranian MDV sequence detected from a layer farm with MD. UT-PCR-9380 was located with vv MDV from Japan and Colombia. Therefore, the relatively high rate of Meq in the unvaccinated broiler farms constitutes support for vaccination. These findings provide the basis for molecular surveillance and further study of MDV mutants and strategies for managing MD in Iran.
| Published in | International Journal of Microbiology and Biotechnology (Volume 8, Issue 3) |
| DOI | 10.11648/j.ijmb.20230803.11 |
| Page(s) | 48-53 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2023. Published by Science Publishing Group |
Marek Disease, Iran, Broiler, Phylogenetic Analysis, Meq
| [1] | Murata S, et al. Genetic characterization of a Marek’s disease virus strain isolated in Japan. Virology journal 2020; 17 (1): 1-13. |
| [2] | Witter R L. Control Strategies for Marek's Disease: A Perspective for the Future. Poultry Sci 1998; 77: 1197-1203. |
| [3] | Qian Z, et al. Transactivation activity of Meq, a Marek's disease herpesvirus bZIP protein persistently expressed in latently infected transformed T cells. Journal of virology 1995; 69 (7): 4037-4044. |
| [4] | Chang KS, Ohashi K, Onuma M. Suppression of transcription activity of the MEQ protein of oncogenic Marek's disease virus serotype 1 (MDV1) by L-MEQ of non-oncogenic MDV1. Journal of veterinary medical science 2002; 64 (12): 1091-1095. |
| [5] | Mescolini G, et al. Molecular characterization of the meq gene of Marek's disease viruses detected in unvaccinated backyard chickens reveals the circulation of low-and high-virulence strains. Poultry Science 2019; 98 (8): 3130-3137. |
| [6] | Mescolini G, et al. Marek's disease viruses circulating in commercial poultry in Italy in the years 2015–2018 are closely related by their meq gene phylogeny. Transboundary and emerging diseases 2020; 67 (1): 98-107. |
| [7] | Gimerno IM, Witter RL, Fadly AM, Sylva RF. New criteria for the diagnosis of Marek' s disease virus-induced lymphoma. Avian pathalogy 2005; 34 (4): 332-340. |
| [8] | Wajid SJ, et al. Prevalence of Marek's disease virus in different chicken populations in Iraq and indicative virulence based on sequence variation in the EcoRI-Q (meq) gene. Avian diseases 2013; 57 (2s1): 562-568. |
| [9] | Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular biology and evolution 2016; 33 (7): 1870-1874. |
| [10] | Ghalyanchilangeroudi A, Hosseini H, Nazarpak HH, Molouki A, Dezfoulian O, Morshed R. Molecular characterization and phylogenetic analysis of marek's disease virus in Iran. Avian Diseases 2022; 66 (3): 1-5. |
| [11] | Davison F and Nair V. Marek's disease: an evolving problem. Elsivier academic press, London, UK 2004; 170, 188. |
| [12] | Farhood M, Toroghi R, Bassami MR, Kianzadeh M, Charkhkar S. Marek's diseases in broiler 's chicken flocks of Khorasan, Isfahan, Tehran provinces. Journal of Comparative Pathobiology 2007; 4 (17): 133-138. |
| [13] | Hablolvarid M. Investigation on Incidence of Marek's Disease in Broiler Flocks of some Regions in Tehran Province, Iran. Archives of Razi Institute 2011; 66 (2): 109-114. |
| [14] | Doosti A and Golshan M. Molecular study for detection of Mareks disease virus (MDV) in southwest of Iran. Scientific Research and Essays 2011; 6 (12): 2560-2563. |
| [15] | Lee SI, Takagi M, Ohashi K, Sugimoto C, Onuma M. Difference in the meq gene between oncogenic and attenuated strains of Marek’s disease virus serotype 1. Journal of Veterinary Medical Science 2000; 62 (3): 287-292. |
APA Style
Rima Morshed, Hossein Hosseini, Naser Sadri, Zahra Ziafati Kafi, Soroush Sarmadi, et al. (2023). Marek’s Disease in Broiler Farms, Iran, 2021: The Phylogenetic Study. International Journal of Microbiology and Biotechnology, 8(3), 48-53. https://doi.org/10.11648/j.ijmb.20230803.11
ACS Style
Rima Morshed; Hossein Hosseini; Naser Sadri; Zahra Ziafati Kafi; Soroush Sarmadi, et al. Marek’s Disease in Broiler Farms, Iran, 2021: The Phylogenetic Study. Int. J. Microbiol. Biotechnol. 2023, 8(3), 48-53. doi: 10.11648/j.ijmb.20230803.11
AMA Style
Rima Morshed, Hossein Hosseini, Naser Sadri, Zahra Ziafati Kafi, Soroush Sarmadi, et al. Marek’s Disease in Broiler Farms, Iran, 2021: The Phylogenetic Study. Int J Microbiol Biotechnol. 2023;8(3):48-53. doi: 10.11648/j.ijmb.20230803.11
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.ijmb.20230803.11,
author = {Rima Morshed and Hossein Hosseini and Naser Sadri and Zahra Ziafati Kafi and Soroush Sarmadi and Nazanin Sarvian and Seyed Hesamodin Emadi Chashmi and Farhad Arab Baghi and Arash Ghalyanchilangeroudi},
title = {Marek’s Disease in Broiler Farms, Iran, 2021: The Phylogenetic Study},
journal = {International Journal of Microbiology and Biotechnology},
volume = {8},
number = {3},
pages = {48-53},
doi = {10.11648/j.ijmb.20230803.11},
url = {https://doi.org/10.11648/j.ijmb.20230803.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20230803.11},
abstract = {Marek's disease virus (MDV) is a highly cell-associated oncogenic alphaherpesvirus that causes chicken T-cell lymphoma. MD is currently controlled by vaccination; however, MDV strains tend to develop increased virulence. Distinct diversity and point mutations are present in the Meq proteins, the major oncoproteins of MDV, suggesting that changes in protein function induced by amino acid substitutions might affect MDV virulence. This study sampled 30 commercial broiler flocks from different Provinces (10 spleens from each flock) at slaughter. Gallid alphaherpesvirus 2 was identified in PCR (using gb primer) of spleen samples of 11 flocks (36.67%). Two provinces, Azerbaijan Gharbi (44%) and Golestan (25%), recorded the highest and lowest infection rates. The oncogene Meq of some positive samples was amplified by PCR and sequenced. MDV strains detected in this study could be put in three branches, with molecular features consistent with virulent and very virulent previously identified MDV. UT-PCR9303 was located with an Iraqi isolate. UT-PCR-9231 had high homology with an Iranian MDV sequence detected from a layer farm with MD. UT-PCR-9380 was located with vv MDV from Japan and Colombia. Therefore, the relatively high rate of Meq in the unvaccinated broiler farms constitutes support for vaccination. These findings provide the basis for molecular surveillance and further study of MDV mutants and strategies for managing MD in Iran.},
year = {2023}
}
TY - JOUR T1 - Marek’s Disease in Broiler Farms, Iran, 2021: The Phylogenetic Study AU - Rima Morshed AU - Hossein Hosseini AU - Naser Sadri AU - Zahra Ziafati Kafi AU - Soroush Sarmadi AU - Nazanin Sarvian AU - Seyed Hesamodin Emadi Chashmi AU - Farhad Arab Baghi AU - Arash Ghalyanchilangeroudi Y1 - 2023/07/31 PY - 2023 N1 - https://doi.org/10.11648/j.ijmb.20230803.11 DO - 10.11648/j.ijmb.20230803.11 T2 - International Journal of Microbiology and Biotechnology JF - International Journal of Microbiology and Biotechnology JO - International Journal of Microbiology and Biotechnology SP - 48 EP - 53 PB - Science Publishing Group SN - 2578-9686 UR - https://doi.org/10.11648/j.ijmb.20230803.11 AB - Marek's disease virus (MDV) is a highly cell-associated oncogenic alphaherpesvirus that causes chicken T-cell lymphoma. MD is currently controlled by vaccination; however, MDV strains tend to develop increased virulence. Distinct diversity and point mutations are present in the Meq proteins, the major oncoproteins of MDV, suggesting that changes in protein function induced by amino acid substitutions might affect MDV virulence. This study sampled 30 commercial broiler flocks from different Provinces (10 spleens from each flock) at slaughter. Gallid alphaherpesvirus 2 was identified in PCR (using gb primer) of spleen samples of 11 flocks (36.67%). Two provinces, Azerbaijan Gharbi (44%) and Golestan (25%), recorded the highest and lowest infection rates. The oncogene Meq of some positive samples was amplified by PCR and sequenced. MDV strains detected in this study could be put in three branches, with molecular features consistent with virulent and very virulent previously identified MDV. UT-PCR9303 was located with an Iraqi isolate. UT-PCR-9231 had high homology with an Iranian MDV sequence detected from a layer farm with MD. UT-PCR-9380 was located with vv MDV from Japan and Colombia. Therefore, the relatively high rate of Meq in the unvaccinated broiler farms constitutes support for vaccination. These findings provide the basis for molecular surveillance and further study of MDV mutants and strategies for managing MD in Iran. VL - 8 IS - 3 ER -
Email: