Antibiotic Resistance Profile of Gram-Negative Bacilli Isolated from Urinary Tract Infections at Laquintinie Hospital in Douala, Cameroon

Cedric Gueguim; Vanessa Kenny Megam; Serge Damase Oyong Assiene; Pulcherie Ngo Lend; Marthe Viviane Fonkam; Edouard OrlineToukap; Aurelle Yondo; Lucien Honore Etame Sone

doi:doi:10.11648/j.ijmb.20220704.12

Antibiotic Resistance Profile of Gram-Negative Bacilli Isolated from Urinary Tract Infections at Laquintinie Hospital in Douala, Cameroon

Cedric Gueguim, Vanessa Kenny Megam, Serge Damase Oyong Assiene, Pulcherie Ngo Lend, Marthe Viviane Fonkam, Edouard OrlineToukap, Aurelle Yondo, Lucien Honore Etame Sone

Published in International Journal of Microbiology and Biotechnology (Volume 7, Issue 4)

Received: 31 August 2022 Accepted: 16 September 2022 Published: 21 October 2022

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Urinary tract infections, usually caused by bacteria that originate from the intestinal flora or the perineal flora, constitute a major public health problem for Africa in general, especially in Cameroon. Known failures with empiric therapy are becoming increasingly problematic. The same is proven true for the consistent bacterial resistance to antibiotics. The purpose of this study was to determine the frequency of Gram-negative bacterial strains isolated from urine samples at Laquintinie Hospital of Douala and to establish their antibiotic resistance profile. Over a period of three months, four hundred and thirty-six (436) urine samples were taken and analyzed at the bacteriology laboratory of the Laquintinie hospital in Douala. The Bacteria were isolated according to standard methods and identification using the API20E gallery. The antibiotic sensitivity profile of the isolated bacteria was done by the disk diffusion method according to the recommendations of the Antibiogram Committee of the French Society of Microbiology 2018. One hundred and fifty-one (151) bacterial strains were isolated, mostly from women (58.67%) with a male or female (M/F) ratio of 0.7. We noted a predominance of Escherichia coli strains (58.28%), followed by Klebsiella Pneumoniae (22.51%), Proteus mirabilis (4.64%), Pseudomonas aeruginosa (3.97%), Enterobacter cloacae (2.65%), Acinetobacter (1.98%), Enterobacter sakazakii (1.32%) and the rest 4.65%. All the bacterial strains isolated showed strong resistance to beta-lactam (greater than 50%) but were predominantly susceptible to Imipenem (96.39%) and Amikacin (90.47%). Escherichia coli was particularly resistant to beta-lactams such as amoxicillin (87.5%), amoxicillin + clavulanic acid (85.24%), Cefuroxime (65.57%), Cefixime (54.83%) and Ceftriaxone (52.70%). The bacterial strains of Klebsiella pneumoniae obtained a total resistance to amoxicillin (100%), clavulanic acid + amoxicillin (69.23%), Cefixime (68.42%), Ceftriaxone (52.70%), and Cefuroxime (52.00%). Escherichia coli (58.28%) and Klebsiella pneumoniae (22.51%) are the two main bacterial species that are the most isolated. Resistance of Escherichia coli and Klebsiella pneumoniae to antibiotics is a growing phenomenon. Monitoring for bacterial resistance to antibiotics is imperative in our context. Develop more effective prevention strategies, screening, prophylactic isolation.

Published in	International Journal of Microbiology and Biotechnology (Volume 7, Issue 4)
DOI	10.11648/j.ijmb.20220704.12
Page(s)	163-168
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Urinary Tract Infection, Gram-Negative Bacilli, Antibiotic, Sensitivity Profile

References

[1]	Daudon M, Traxer O, Lechevallier E, Saussine C. Épidémiologie des lithiases urinaires. Prog En Urol. 1 déc 2008; 18 (12): 802‑14.
[2]	2SPILF E. Conférence de consensus co-organisée par la Société de pathologie infectieuse de langue française (SPILF) et l’Association française d’urologie (AFU) - Infections urinaires nosocomiales de l’adulte [Internet]. EM-Consulte. 2003 [cité 24 mars 2022]. Disponible sur: https://www.em-consulte.com/article/17322.
[3]	Abdallah B, Noomen S, Ben Elhadj Khélifa A, Sahnoun O, Elargoubi A, Mastouri M. Profil de sensibilité aux antibiotiques des souches de Pseudomonas aeruginosa isolées dans la région de Monastir [Internet]. EM-Consulte. 2008 [cité 24 mars 2022]. Disponible sur: https://www.em-consulte.com/article/187082.
[4]	YALA D, MERAD AS, MOHAMED D, OUAR KORICH MN. Resistance bacterienne aux antibiotiques. Médecine du Maghreb. 2011.
[5]	Sidi BA. Epidémiologie De La Résistance Aux Antibiotiques Des Entérobactéries Au Niveau Du C.h.u [Internet]. 2014 [cité 24 mars 2022]. Disponible sur: https://www.theses-algerie.com.
[6]	Mariko M, Sow DS, Traoré B, Traoré A, Traoré D, Dabo G, et al. Profil Bactériologique de l’Infection Urinaire chez les Diabétiques à l’Hôpital du Mali (Bamako). Health Sci Dis [Internet]. 24 janv 2021 [cité 26 mars 2022]; 22 (2). Disponible sur: https://www.hsd-fmsb.org/index.php/hsd/article/view/2539.
[7]	Zomahoun CINP. Evaluation de la sensibilite aux antibiotiques des bacteries isolees des infections urinaires au Laboratoire de bacteriologie du Centre National Hospitalier Universitaire - Hubert Koutoukou Maga (C. N. H. H.-H. K. M) de Cotonou. A propos de 231 souches bacteriennes isolees du 1er avril au 31 juillet 2004. 2005 [cité 24 mars 2022]; Disponible sur: http://indexmedicus.afro.who.int/iah/fulltext/Thesis_Bamako/05P11.pdf
[8]	Dossim S, Kpatcha TM, Salou M, Azimti A, Dagnra AY, Segbena AY, et al. Infections urinaires communautaires et acquises au Centre Hospitalier Universitaire Campus de Lomé: bacteries en cause. J Rech Sci L’Université Lomé. 2017; 19 (4): 441‑6.
[9]	Zanella M-C, Schoofs F, Huttner B, Huttner A. Infections urinaires basses non associées aux sondes urinaires chez l’homme. Urétrite, cystite et prostatite [Internet]. Revue Medicale Suisse. 2017 [cité 26 mars 2022]. Disponible sur: https://www.revmed.ch/revue-medicale-suisse/2017/revue-medicale-suisse-558/infections-urinaires-basses-non-associees-aux-sondes-urinaires-chez-l-homme.-uretrite-cystite-et-prostatite.
[10]	Ebongue CO, Tsiazok MD, Mefo’o JPN, Ngaba GP, Beyiha G, Adiogo D. Evolution de la résistance aux antibiotiques des entérobactéries isolées à l’Hôpital Général de Douala de 2005 à 2012. Pan Afr Med J [Internet]. 12 mars 2015 [cité 18 sept 2021]; 20 (227). Disponible sur: https://www.panafrican-med-journal.com/content/article/20/227/full.
[11]	Lyonga E, Toukam M, Atashili J, Gonsu H, Adiogo D, Mesembe M, et al. A Comparative Study on Susceptibility of Enterobacteriaceae to Six Quinolones in Yaounde. Health Sci Dis [Internet]. 11 nov 2013 [cité 24 mars 2022]; 14 (4). Disponible sur: https://www.hsd-fmsb.org/index.php/hsd/article/view/193.
[12]	Sekhsokh Y, Chadli M, El Hamzaoui SA. Fréquence et sensibilité aux antibiotiques des bactéries isolées dans les urines. Médecine Mal Infect. 1 juin 2008; 38 (6): 324‑7.
[13]	Ben Haj Khalifa A, Khedher M. Epidémiologie des souches de Klebsiella spp. uropathogènes productrices de β-lactamases à spectre élargi dans un hôpital universitaire Tunisien, 2009 [Internet]. EM-Consulte. 2009 [cité 24 mars 2022]. Disponible sur: https://www.em-consulte.com/article/703974.
[14]	Kassaa IA, Zaylaa M, Hussein S, Omari KE, Chihib N-E, Hamze M, et al. Identification of the first bacteriocin isolated in Lebanon extracted via a modified adsorption-desorption method and its potential food application. Int Arab J Antimicrob Agents [Internet]. 19 mars 2018 [cité 24 mars 2022]; 8 (1). Disponible sur: http://imed.pub/ojs/index.php/IAJAA/article/view/2325.
[15]	Gangoué-Piéboji J, Bedenic B, Koulla-Shiro S, Randegger C, Adiogo D, Ngassam P, et al. Extended-spectrum-beta-lactamase-producing Enterobacteriaceae in Yaounde, Cameroon. J Clin Microbiol. juill 2005; 43 (7): 3273‑7.
[16]	Hanberger H, Garcia-Rodriguez JA, Gobernado M, Goossens H, Nilsson LE, Struelens MJ. Antibiotic susceptibility among aerobic gram-negative bacilli in intensive care units in 5 European countries. French and Portuguese ICU Study Groups. JAMA. 6 janv 1999; 281 (1): 67‑71.
[17]	Prère M-F, Licznar P, Decramer S, Fayet O. Escherichia coli des infections urinaires et pyélonéphrites aiguës en pédiatrie: 1% des souches sont résistantes à certaines céphalosporines de 3e génération. Pathol Biol. 1 oct 2004; 52 (8): 497‑500.
[18]	GUILLEMOT (D.), Effet de l’usage des antibiotiques sur l’évolution des résistances bactériennes; 2001.
[19]	Koeck J-L, Arlet G, Philippon A, Basmaciogullari S, Thien HV, Buisson Y, et al. A plasmid-mediated CMY-2 β-lactamase from an Algerian clinical isolate of Salmonella senftenberg. FEMS Microbiol Lett. 1 juill 1997; 152 (2): 255‑60.
[20]	Seck A. Maladie ulcereuse gastro-duodenale (MUGD) et Helicobacter pylori (Hp) : profil de sensibilité des souches de Hp isolées à Dakar. [Dakar]: Université CHEICK ANTA DIOP; 2001.
[21]	Tsolaki V, Mantzarlis K, Mpakalis A, Malli E, Tsimpoukas F, Tsirogianni A, et al. Ceftazidime-Avibactam To Treat Life-Threatening Infections by Carbapenem-Resistant Pathogens in Critically Ill Mechanically Ventilated Patients. Antimicrob Agents Chemother. 21 févr 2020; 64 (3): e02320-19.
[22]	Moubareck C, Doucet-Populaire F, Hamze M, Daoud Z, Weill F-X. First Extended-Spectrum-β-Lactamase (CTX-M-15) -Producing Salmonella enterica Serotype Typhimurium Isolate Identified in Lebanon. Antimicrob Agents Chemother. févr 2005; 49 (2): 864‑5.
[23]	Lee S, Park Y-J, Kim M, Lee HK, Han K, Kang CS, et al. Prevalence of Ambler class A and D β-lactamases among clinical isolates of Pseudomonas aeruginosa in Korea. J Antimicrob Chemother. 1 juill 2005; 56 (1): 122‑7.
[24]	Duszynska W, Taccone FS, Hurkacz M, Kowalska-Krochmal B, Wiela-Hojeńska A, Kübler A. Therapeutic drug monitoring of amikacin in septic patients. Crit Care. 25 juill 2013; 17 (4): R165.
[25]	Spanu T, Luzzaro F, Perilli M, Amicosante G, Toniolo A, Fadda G, et al. Occurrence of Extended-Spectrum β-Lactamases in Members of the Family Enterobacteriaceae in Italy: Implications for Resistance to β-Lactams and Other Antimicrobial Drugs. Antimicrob Agents Chemother. janv 2002; 46 (1): 196‑202.
[26]	Larabi K, Masmoudi A, Fendri C. Étude bactériologique et phénotypes de résistance des germes responsables d’infections urinaires dans un CHU de Tunis : à propos de 1930 cas. 2003.
[27]	Rice EW, Clark RM, Johnson CH. Chlorine inactivation of Escherichia coli O157:H7. Emerg Infect Dis. 1999; 5 (3): 461‑3.
[28]	Jacobson KL, Cohen SH, Inciardi JF, King JH, Lippert WE, Iglesias T, et al. The relationship between antecedent antibiotic use and resistance to extended-spectrum cephalosporins in group I beta-lactamase-producing organisms. Publ Infect Dis. nov 1995; 21 (5): 1107‑13.
[29]	Pugsley MP, Dworzack DL, Horowitz EA, Cuevas TA, Sanders WE Jr, Sanders CC. Efficacy of Ciprofloxacin in the Treatment of Nasopharyngeal Carriers of Neisseria meningitidis. J Infect Dis. 1 juill 1987; 156 (1): 211‑3.

Cite This Article

Plain Text BibTeX RIS

APA Style

Cedric Gueguim, Vanessa Kenny Megam, Serge Damase Oyong Assiene, Pulcherie Ngo Lend, Marthe Viviane Fonkam, et al. (2022). Antibiotic Resistance Profile of Gram-Negative Bacilli Isolated from Urinary Tract Infections at Laquintinie Hospital in Douala, Cameroon. International Journal of Microbiology and Biotechnology, 7(4), 163-168. https://doi.org/10.11648/j.ijmb.20220704.12

Copy | Download

ACS Style

Cedric Gueguim; Vanessa Kenny Megam; Serge Damase Oyong Assiene; Pulcherie Ngo Lend; Marthe Viviane Fonkam, et al. Antibiotic Resistance Profile of Gram-Negative Bacilli Isolated from Urinary Tract Infections at Laquintinie Hospital in Douala, Cameroon. Int. J. Microbiol. Biotechnol. 2022, 7(4), 163-168. doi: 10.11648/j.ijmb.20220704.12

Copy | Download

AMA Style

Cedric Gueguim, Vanessa Kenny Megam, Serge Damase Oyong Assiene, Pulcherie Ngo Lend, Marthe Viviane Fonkam, et al. Antibiotic Resistance Profile of Gram-Negative Bacilli Isolated from Urinary Tract Infections at Laquintinie Hospital in Douala, Cameroon. Int J Microbiol Biotechnol. 2022;7(4):163-168. doi: 10.11648/j.ijmb.20220704.12

Copy | Download

@article{10.11648/j.ijmb.20220704.12,
  author = {Cedric Gueguim and Vanessa Kenny Megam and Serge Damase Oyong Assiene and Pulcherie Ngo Lend and Marthe Viviane Fonkam and Edouard OrlineToukap and Aurelle Yondo and Lucien Honore Etame Sone},
  title = {Antibiotic Resistance Profile of Gram-Negative Bacilli Isolated from Urinary Tract Infections at Laquintinie Hospital in Douala, Cameroon},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {7},
  number = {4},
  pages = {163-168},
  doi = {10.11648/j.ijmb.20220704.12},
  url = {https://doi.org/10.11648/j.ijmb.20220704.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20220704.12},
  abstract = {Urinary tract infections, usually caused by bacteria that originate from the intestinal flora or the perineal flora, constitute a major public health problem for Africa in general, especially in Cameroon. Known failures with empiric therapy are becoming increasingly problematic. The same is proven true for the consistent bacterial resistance to antibiotics. The purpose of this study was to determine the frequency of Gram-negative bacterial strains isolated from urine samples at Laquintinie Hospital of Douala and to establish their antibiotic resistance profile. Over a period of three months, four hundred and thirty-six (436) urine samples were taken and analyzed at the bacteriology laboratory of the Laquintinie hospital in Douala. The Bacteria were isolated according to standard methods and identification using the API20E gallery. The antibiotic sensitivity profile of the isolated bacteria was done by the disk diffusion method according to the recommendations of the Antibiogram Committee of the French Society of Microbiology 2018. One hundred and fifty-one (151) bacterial strains were isolated, mostly from women (58.67%) with a male or female (M/F) ratio of 0.7. We noted a predominance of Escherichia coli strains (58.28%), followed by Klebsiella Pneumoniae (22.51%), Proteus mirabilis (4.64%), Pseudomonas aeruginosa (3.97%), Enterobacter cloacae (2.65%), Acinetobacter (1.98%), Enterobacter sakazakii (1.32%) and the rest 4.65%. All the bacterial strains isolated showed strong resistance to beta-lactam (greater than 50%) but were predominantly susceptible to Imipenem (96.39%) and Amikacin (90.47%). Escherichia coli was particularly resistant to beta-lactams such as amoxicillin (87.5%), amoxicillin + clavulanic acid (85.24%), Cefuroxime (65.57%), Cefixime (54.83%) and Ceftriaxone (52.70%). The bacterial strains of Klebsiella pneumoniae obtained a total resistance to amoxicillin (100%), clavulanic acid + amoxicillin (69.23%), Cefixime (68.42%), Ceftriaxone (52.70%), and Cefuroxime (52.00%). Escherichia coli (58.28%) and Klebsiella pneumoniae (22.51%) are the two main bacterial species that are the most isolated. Resistance of Escherichia coli and Klebsiella pneumoniae to antibiotics is a growing phenomenon. Monitoring for bacterial resistance to antibiotics is imperative in our context. Develop more effective prevention strategies, screening, prophylactic isolation.},
 year = {2022}
}

Copy | Download

TY  - JOUR
T1  - Antibiotic Resistance Profile of Gram-Negative Bacilli Isolated from Urinary Tract Infections at Laquintinie Hospital in Douala, Cameroon
AU  - Cedric Gueguim
AU  - Vanessa Kenny Megam
AU  - Serge Damase Oyong Assiene
AU  - Pulcherie Ngo Lend
AU  - Marthe Viviane Fonkam
AU  - Edouard OrlineToukap
AU  - Aurelle Yondo
AU  - Lucien Honore Etame Sone
Y1  - 2022/10/21
PY  - 2022
N1  - https://doi.org/10.11648/j.ijmb.20220704.12
DO  - 10.11648/j.ijmb.20220704.12
T2  - International Journal of Microbiology and Biotechnology
JF  - International Journal of Microbiology and Biotechnology
JO  - International Journal of Microbiology and Biotechnology
SP  - 163
EP  - 168
PB  - Science Publishing Group
SN  - 2578-9686
UR  - https://doi.org/10.11648/j.ijmb.20220704.12
AB  - Urinary tract infections, usually caused by bacteria that originate from the intestinal flora or the perineal flora, constitute a major public health problem for Africa in general, especially in Cameroon. Known failures with empiric therapy are becoming increasingly problematic. The same is proven true for the consistent bacterial resistance to antibiotics. The purpose of this study was to determine the frequency of Gram-negative bacterial strains isolated from urine samples at Laquintinie Hospital of Douala and to establish their antibiotic resistance profile. Over a period of three months, four hundred and thirty-six (436) urine samples were taken and analyzed at the bacteriology laboratory of the Laquintinie hospital in Douala. The Bacteria were isolated according to standard methods and identification using the API20E gallery. The antibiotic sensitivity profile of the isolated bacteria was done by the disk diffusion method according to the recommendations of the Antibiogram Committee of the French Society of Microbiology 2018. One hundred and fifty-one (151) bacterial strains were isolated, mostly from women (58.67%) with a male or female (M/F) ratio of 0.7. We noted a predominance of Escherichia coli strains (58.28%), followed by Klebsiella Pneumoniae (22.51%), Proteus mirabilis (4.64%), Pseudomonas aeruginosa (3.97%), Enterobacter cloacae (2.65%), Acinetobacter (1.98%), Enterobacter sakazakii (1.32%) and the rest 4.65%. All the bacterial strains isolated showed strong resistance to beta-lactam (greater than 50%) but were predominantly susceptible to Imipenem (96.39%) and Amikacin (90.47%). Escherichia coli was particularly resistant to beta-lactams such as amoxicillin (87.5%), amoxicillin + clavulanic acid (85.24%), Cefuroxime (65.57%), Cefixime (54.83%) and Ceftriaxone (52.70%). The bacterial strains of Klebsiella pneumoniae obtained a total resistance to amoxicillin (100%), clavulanic acid + amoxicillin (69.23%), Cefixime (68.42%), Ceftriaxone (52.70%), and Cefuroxime (52.00%). Escherichia coli (58.28%) and Klebsiella pneumoniae (22.51%) are the two main bacterial species that are the most isolated. Resistance of Escherichia coli and Klebsiella pneumoniae to antibiotics is a growing phenomenon. Monitoring for bacterial resistance to antibiotics is imperative in our context. Develop more effective prevention strategies, screening, prophylactic isolation.
VL  - 7
IS  - 4
ER  -

Copy | Download

Author Information

Cedric Gueguim

Department of Biology, Higher Institute of Medical Technology, Yaounde, Cameroon
Vanessa Kenny Megam

School of Health Sciences, Catholic University of Central Africa, Yaounde, Cameroon
Serge Damase Oyong Assiene

Faculty of Medicine and Pharmaceutical Sciences, University of Douala, Douala, Cameroon
Pulcherie Ngo Lend

Central Laboratory, Laquintinie Hospital, Douala, Cameroon
Marthe Viviane Fonkam

School of Health Sciences, Catholic University of Central Africa, Yaounde, Cameroon
Edouard OrlineToukap

School of Health Sciences, Catholic University of Central Africa, Yaounde, Cameroon
Aurelle Yondo

College of Veterinary Medicine, The University of Georgia, Athens, Georgia
Lucien Honore Etame Sone

Department of Biology, Higher Institute of Medical Technology, Yaounde, Cameroon

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Cedric Gueguim, Vanessa Kenny Megam, Serge Damase Oyong Assiene, Pulcherie Ngo Lend, Marthe Viviane Fonkam, et al. (2022). Antibiotic Resistance Profile of Gram-Negative Bacilli Isolated from Urinary Tract Infections at Laquintinie Hospital in Douala, Cameroon. International Journal of Microbiology and Biotechnology, 7(4), 163-168. https://doi.org/10.11648/j.ijmb.20220704.12

Copy | Download

ACS Style

Cedric Gueguim; Vanessa Kenny Megam; Serge Damase Oyong Assiene; Pulcherie Ngo Lend; Marthe Viviane Fonkam, et al. Antibiotic Resistance Profile of Gram-Negative Bacilli Isolated from Urinary Tract Infections at Laquintinie Hospital in Douala, Cameroon. Int. J. Microbiol. Biotechnol. 2022, 7(4), 163-168. doi: 10.11648/j.ijmb.20220704.12

Copy | Download

AMA Style

Cedric Gueguim, Vanessa Kenny Megam, Serge Damase Oyong Assiene, Pulcherie Ngo Lend, Marthe Viviane Fonkam, et al. Antibiotic Resistance Profile of Gram-Negative Bacilli Isolated from Urinary Tract Infections at Laquintinie Hospital in Douala, Cameroon. Int J Microbiol Biotechnol. 2022;7(4):163-168. doi: 10.11648/j.ijmb.20220704.12

Copy | Download

@article{10.11648/j.ijmb.20220704.12,
  author = {Cedric Gueguim and Vanessa Kenny Megam and Serge Damase Oyong Assiene and Pulcherie Ngo Lend and Marthe Viviane Fonkam and Edouard OrlineToukap and Aurelle Yondo and Lucien Honore Etame Sone},
  title = {Antibiotic Resistance Profile of Gram-Negative Bacilli Isolated from Urinary Tract Infections at Laquintinie Hospital in Douala, Cameroon},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {7},
  number = {4},
  pages = {163-168},
  doi = {10.11648/j.ijmb.20220704.12},
  url = {https://doi.org/10.11648/j.ijmb.20220704.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20220704.12},
  abstract = {Urinary tract infections, usually caused by bacteria that originate from the intestinal flora or the perineal flora, constitute a major public health problem for Africa in general, especially in Cameroon. Known failures with empiric therapy are becoming increasingly problematic. The same is proven true for the consistent bacterial resistance to antibiotics. The purpose of this study was to determine the frequency of Gram-negative bacterial strains isolated from urine samples at Laquintinie Hospital of Douala and to establish their antibiotic resistance profile. Over a period of three months, four hundred and thirty-six (436) urine samples were taken and analyzed at the bacteriology laboratory of the Laquintinie hospital in Douala. The Bacteria were isolated according to standard methods and identification using the API20E gallery. The antibiotic sensitivity profile of the isolated bacteria was done by the disk diffusion method according to the recommendations of the Antibiogram Committee of the French Society of Microbiology 2018. One hundred and fifty-one (151) bacterial strains were isolated, mostly from women (58.67%) with a male or female (M/F) ratio of 0.7. We noted a predominance of Escherichia coli strains (58.28%), followed by Klebsiella Pneumoniae (22.51%), Proteus mirabilis (4.64%), Pseudomonas aeruginosa (3.97%), Enterobacter cloacae (2.65%), Acinetobacter (1.98%), Enterobacter sakazakii (1.32%) and the rest 4.65%. All the bacterial strains isolated showed strong resistance to beta-lactam (greater than 50%) but were predominantly susceptible to Imipenem (96.39%) and Amikacin (90.47%). Escherichia coli was particularly resistant to beta-lactams such as amoxicillin (87.5%), amoxicillin + clavulanic acid (85.24%), Cefuroxime (65.57%), Cefixime (54.83%) and Ceftriaxone (52.70%). The bacterial strains of Klebsiella pneumoniae obtained a total resistance to amoxicillin (100%), clavulanic acid + amoxicillin (69.23%), Cefixime (68.42%), Ceftriaxone (52.70%), and Cefuroxime (52.00%). Escherichia coli (58.28%) and Klebsiella pneumoniae (22.51%) are the two main bacterial species that are the most isolated. Resistance of Escherichia coli and Klebsiella pneumoniae to antibiotics is a growing phenomenon. Monitoring for bacterial resistance to antibiotics is imperative in our context. Develop more effective prevention strategies, screening, prophylactic isolation.},
 year = {2022}
}

Copy | Download

TY  - JOUR
T1  - Antibiotic Resistance Profile of Gram-Negative Bacilli Isolated from Urinary Tract Infections at Laquintinie Hospital in Douala, Cameroon
AU  - Cedric Gueguim
AU  - Vanessa Kenny Megam
AU  - Serge Damase Oyong Assiene
AU  - Pulcherie Ngo Lend
AU  - Marthe Viviane Fonkam
AU  - Edouard OrlineToukap
AU  - Aurelle Yondo
AU  - Lucien Honore Etame Sone
Y1  - 2022/10/21
PY  - 2022
N1  - https://doi.org/10.11648/j.ijmb.20220704.12
DO  - 10.11648/j.ijmb.20220704.12
T2  - International Journal of Microbiology and Biotechnology
JF  - International Journal of Microbiology and Biotechnology
JO  - International Journal of Microbiology and Biotechnology
SP  - 163
EP  - 168
PB  - Science Publishing Group
SN  - 2578-9686
UR  - https://doi.org/10.11648/j.ijmb.20220704.12
AB  - Urinary tract infections, usually caused by bacteria that originate from the intestinal flora or the perineal flora, constitute a major public health problem for Africa in general, especially in Cameroon. Known failures with empiric therapy are becoming increasingly problematic. The same is proven true for the consistent bacterial resistance to antibiotics. The purpose of this study was to determine the frequency of Gram-negative bacterial strains isolated from urine samples at Laquintinie Hospital of Douala and to establish their antibiotic resistance profile. Over a period of three months, four hundred and thirty-six (436) urine samples were taken and analyzed at the bacteriology laboratory of the Laquintinie hospital in Douala. The Bacteria were isolated according to standard methods and identification using the API20E gallery. The antibiotic sensitivity profile of the isolated bacteria was done by the disk diffusion method according to the recommendations of the Antibiogram Committee of the French Society of Microbiology 2018. One hundred and fifty-one (151) bacterial strains were isolated, mostly from women (58.67%) with a male or female (M/F) ratio of 0.7. We noted a predominance of Escherichia coli strains (58.28%), followed by Klebsiella Pneumoniae (22.51%), Proteus mirabilis (4.64%), Pseudomonas aeruginosa (3.97%), Enterobacter cloacae (2.65%), Acinetobacter (1.98%), Enterobacter sakazakii (1.32%) and the rest 4.65%. All the bacterial strains isolated showed strong resistance to beta-lactam (greater than 50%) but were predominantly susceptible to Imipenem (96.39%) and Amikacin (90.47%). Escherichia coli was particularly resistant to beta-lactams such as amoxicillin (87.5%), amoxicillin + clavulanic acid (85.24%), Cefuroxime (65.57%), Cefixime (54.83%) and Ceftriaxone (52.70%). The bacterial strains of Klebsiella pneumoniae obtained a total resistance to amoxicillin (100%), clavulanic acid + amoxicillin (69.23%), Cefixime (68.42%), Ceftriaxone (52.70%), and Cefuroxime (52.00%). Escherichia coli (58.28%) and Klebsiella pneumoniae (22.51%) are the two main bacterial species that are the most isolated. Resistance of Escherichia coli and Klebsiella pneumoniae to antibiotics is a growing phenomenon. Monitoring for bacterial resistance to antibiotics is imperative in our context. Develop more effective prevention strategies, screening, prophylactic isolation.
VL  - 7
IS  - 4
ER  -

Copy | Download