Isolation and propagation of influenza virus from Influenza Like Illness (ILI) clinical sample is essential for the surveillance of circulating virus, such as antigenic and genetic analyses, antiviral sensitivity surveillance, as well as annual influenza vaccine selection. Madin-Darby canine kidney (MDCK) cell is conventionally used for virus isolation in public health laboratories. Throat swap samples of Influenza like Illness (ILI) were collected from two sentinel hospitals and screened seasonal influenza by real-time reverse transcription polymerase chain reaction (RT–PCR). H3N2 positive samples were performed virus isolation in MDCK cells. Samples were stored under different conditions before inoculation, 1-2 days at 2-8°C, 4-5 day or 8-9 days at 2-8°C, and no less than two months at -80°C. The results showed that long term (>2 month) -80°C storage of clinical samples (15.12%) had significantly lower virus isolation rate compare to short term (1-2 days and 4-9 days) under 2-8°C storage (88.37% for 1-2 days and 52.33% for 4-9 days). For those samples stored at 4°C, the shorter of the storage time, the better of sample quality and virus activity could be obtained, resulting in higher isolation rate. This study provides evidence for influenza surveillance and sample quality control.
| Published in | International Journal of Microbiology and Biotechnology (Volume 7, Issue 1) |
| DOI | 10.11648/j.ijmb.20220701.14 |
| Page(s) | 31-36 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
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Copyright © The Author(s), 2022. Published by Science Publishing Group |
Influenza, Virus Isolation, Sample Quality, Sample Storage Condition
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APA Style
Hongyan Bai, Jiachen Zhao, Chunna Ma, Hejiang Wei, Xiyan Li, et al. (2022). Virus Isolation and Propagation from H3N2 Influenza Infected Human Clinical Samples Under Distinct Sample Storage Conditions. International Journal of Microbiology and Biotechnology, 7(1), 31-36. https://doi.org/10.11648/j.ijmb.20220701.14
ACS Style
Hongyan Bai; Jiachen Zhao; Chunna Ma; Hejiang Wei; Xiyan Li, et al. Virus Isolation and Propagation from H3N2 Influenza Infected Human Clinical Samples Under Distinct Sample Storage Conditions. Int. J. Microbiol. Biotechnol. 2022, 7(1), 31-36. doi: 10.11648/j.ijmb.20220701.14
AMA Style
Hongyan Bai, Jiachen Zhao, Chunna Ma, Hejiang Wei, Xiyan Li, et al. Virus Isolation and Propagation from H3N2 Influenza Infected Human Clinical Samples Under Distinct Sample Storage Conditions. Int J Microbiol Biotechnol. 2022;7(1):31-36. doi: 10.11648/j.ijmb.20220701.14
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Download@article{10.11648/j.ijmb.20220701.14,
author = {Hongyan Bai and Jiachen Zhao and Chunna Ma and Hejiang Wei and Xiyan Li and Qiongqiong Fang and Peng Yang and Quanyi Wang and Dayan Wang and Li Xin},
title = {Virus Isolation and Propagation from H3N2 Influenza Infected Human Clinical Samples Under Distinct Sample Storage Conditions},
journal = {International Journal of Microbiology and Biotechnology},
volume = {7},
number = {1},
pages = {31-36},
doi = {10.11648/j.ijmb.20220701.14},
url = {https://doi.org/10.11648/j.ijmb.20220701.14},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20220701.14},
abstract = {Isolation and propagation of influenza virus from Influenza Like Illness (ILI) clinical sample is essential for the surveillance of circulating virus, such as antigenic and genetic analyses, antiviral sensitivity surveillance, as well as annual influenza vaccine selection. Madin-Darby canine kidney (MDCK) cell is conventionally used for virus isolation in public health laboratories. Throat swap samples of Influenza like Illness (ILI) were collected from two sentinel hospitals and screened seasonal influenza by real-time reverse transcription polymerase chain reaction (RT–PCR). H3N2 positive samples were performed virus isolation in MDCK cells. Samples were stored under different conditions before inoculation, 1-2 days at 2-8°C, 4-5 day or 8-9 days at 2-8°C, and no less than two months at -80°C. The results showed that long term (>2 month) -80°C storage of clinical samples (15.12%) had significantly lower virus isolation rate compare to short term (1-2 days and 4-9 days) under 2-8°C storage (88.37% for 1-2 days and 52.33% for 4-9 days). For those samples stored at 4°C, the shorter of the storage time, the better of sample quality and virus activity could be obtained, resulting in higher isolation rate. This study provides evidence for influenza surveillance and sample quality control.},
year = {2022}
}
TY - JOUR T1 - Virus Isolation and Propagation from H3N2 Influenza Infected Human Clinical Samples Under Distinct Sample Storage Conditions AU - Hongyan Bai AU - Jiachen Zhao AU - Chunna Ma AU - Hejiang Wei AU - Xiyan Li AU - Qiongqiong Fang AU - Peng Yang AU - Quanyi Wang AU - Dayan Wang AU - Li Xin Y1 - 2022/03/09 PY - 2022 N1 - https://doi.org/10.11648/j.ijmb.20220701.14 DO - 10.11648/j.ijmb.20220701.14 T2 - International Journal of Microbiology and Biotechnology JF - International Journal of Microbiology and Biotechnology JO - International Journal of Microbiology and Biotechnology SP - 31 EP - 36 PB - Science Publishing Group SN - 2578-9686 UR - https://doi.org/10.11648/j.ijmb.20220701.14 AB - Isolation and propagation of influenza virus from Influenza Like Illness (ILI) clinical sample is essential for the surveillance of circulating virus, such as antigenic and genetic analyses, antiviral sensitivity surveillance, as well as annual influenza vaccine selection. Madin-Darby canine kidney (MDCK) cell is conventionally used for virus isolation in public health laboratories. Throat swap samples of Influenza like Illness (ILI) were collected from two sentinel hospitals and screened seasonal influenza by real-time reverse transcription polymerase chain reaction (RT–PCR). H3N2 positive samples were performed virus isolation in MDCK cells. Samples were stored under different conditions before inoculation, 1-2 days at 2-8°C, 4-5 day or 8-9 days at 2-8°C, and no less than two months at -80°C. The results showed that long term (>2 month) -80°C storage of clinical samples (15.12%) had significantly lower virus isolation rate compare to short term (1-2 days and 4-9 days) under 2-8°C storage (88.37% for 1-2 days and 52.33% for 4-9 days). For those samples stored at 4°C, the shorter of the storage time, the better of sample quality and virus activity could be obtained, resulting in higher isolation rate. This study provides evidence for influenza surveillance and sample quality control. VL - 7 IS - 1 ER -
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