Marine microorganisms are the important resources for natural drug discovery. However, most of genes are usually in silent and could not express under the condition of traditional culture, which limits the discovery of lead compounds. In the present research, marine fungus Schizophyllum sp. YS-08 was selected as the research object, which was from Yellow Sea of China, to activate its silent genes by changing the medium of nutrients and environmental conditions. The crude extracts from metabolites of marine fungi were analyzed by HPLC, and their antioxidant activity and acetylcholinesterase (AChE) inhibitory activity were studied by DPPH radical-scavenging and Ellman's method, respectively. The results indicated that metabolic pathway of fungus was regulated effectively in different culture conditions, especially in the normal sea water medium with peptone. The antioxidant activity and AChE inhibitory activity increased significantly in comparison with wild strain and increase of the quantity of chemical constituents were even more. It is also observed that the strains showed relatively slow growth in the high salinity situation, while this adversely environmental condition could promote and produce more active metabolites. Furthermore, 6 mutant strains were obtained under salt stress and were identified. Interestingly, we found that all mutant strains had potency toward antioxidant and AChE inhibitory activity. Among them, YS-08-2 was the most potent, scavenging more than 56.02% towards DPPH free radicals, and YS-08-1, YS-08-4 and YS-08-5 exhibited more than 40% inhibition against AChE. HPLC analysis of extracts showed the metabolites of most fungi were more abundant after regulation of culture conditions.
| Published in | International Journal of Microbiology and Biotechnology (Volume 6, Issue 3) |
| DOI | 10.11648/j.ijmb.20210603.13 |
| Page(s) | 78-85 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2021. Published by Science Publishing Group |
Symbiotic Fungi, OSMAC Strategy, Secondary Metabolites, Antioxidant Activity, AChE Inhibitory Activity
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APA Style
Huannan Wang, Maocai Yan, Mengdi Liang, Xiujian Wei, Zhao Shang, et al. (2021). Regulation and Activity Evaluation of Secondary Metabolism of an Oyster Symbiotic Fungus Schizophyllum sp. YS-08. International Journal of Microbiology and Biotechnology, 6(3), 78-85. https://doi.org/10.11648/j.ijmb.20210603.13
ACS Style
Huannan Wang; Maocai Yan; Mengdi Liang; Xiujian Wei; Zhao Shang, et al. Regulation and Activity Evaluation of Secondary Metabolism of an Oyster Symbiotic Fungus Schizophyllum sp. YS-08. Int. J. Microbiol. Biotechnol. 2021, 6(3), 78-85. doi: 10.11648/j.ijmb.20210603.13
AMA Style
Huannan Wang, Maocai Yan, Mengdi Liang, Xiujian Wei, Zhao Shang, et al. Regulation and Activity Evaluation of Secondary Metabolism of an Oyster Symbiotic Fungus Schizophyllum sp. YS-08. Int J Microbiol Biotechnol. 2021;6(3):78-85. doi: 10.11648/j.ijmb.20210603.13
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Download@article{10.11648/j.ijmb.20210603.13,
author = {Huannan Wang and Maocai Yan and Mengdi Liang and Xiujian Wei and Zhao Shang and Zhen Zhang},
title = {Regulation and Activity Evaluation of Secondary Metabolism of an Oyster Symbiotic Fungus Schizophyllum sp. YS-08},
journal = {International Journal of Microbiology and Biotechnology},
volume = {6},
number = {3},
pages = {78-85},
doi = {10.11648/j.ijmb.20210603.13},
url = {https://doi.org/10.11648/j.ijmb.20210603.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20210603.13},
abstract = {Marine microorganisms are the important resources for natural drug discovery. However, most of genes are usually in silent and could not express under the condition of traditional culture, which limits the discovery of lead compounds. In the present research, marine fungus Schizophyllum sp. YS-08 was selected as the research object, which was from Yellow Sea of China, to activate its silent genes by changing the medium of nutrients and environmental conditions. The crude extracts from metabolites of marine fungi were analyzed by HPLC, and their antioxidant activity and acetylcholinesterase (AChE) inhibitory activity were studied by DPPH radical-scavenging and Ellman's method, respectively. The results indicated that metabolic pathway of fungus was regulated effectively in different culture conditions, especially in the normal sea water medium with peptone. The antioxidant activity and AChE inhibitory activity increased significantly in comparison with wild strain and increase of the quantity of chemical constituents were even more. It is also observed that the strains showed relatively slow growth in the high salinity situation, while this adversely environmental condition could promote and produce more active metabolites. Furthermore, 6 mutant strains were obtained under salt stress and were identified. Interestingly, we found that all mutant strains had potency toward antioxidant and AChE inhibitory activity. Among them, YS-08-2 was the most potent, scavenging more than 56.02% towards DPPH free radicals, and YS-08-1, YS-08-4 and YS-08-5 exhibited more than 40% inhibition against AChE. HPLC analysis of extracts showed the metabolites of most fungi were more abundant after regulation of culture conditions.},
year = {2021}
}
TY - JOUR T1 - Regulation and Activity Evaluation of Secondary Metabolism of an Oyster Symbiotic Fungus Schizophyllum sp. YS-08 AU - Huannan Wang AU - Maocai Yan AU - Mengdi Liang AU - Xiujian Wei AU - Zhao Shang AU - Zhen Zhang Y1 - 2021/08/09 PY - 2021 N1 - https://doi.org/10.11648/j.ijmb.20210603.13 DO - 10.11648/j.ijmb.20210603.13 T2 - International Journal of Microbiology and Biotechnology JF - International Journal of Microbiology and Biotechnology JO - International Journal of Microbiology and Biotechnology SP - 78 EP - 85 PB - Science Publishing Group SN - 2578-9686 UR - https://doi.org/10.11648/j.ijmb.20210603.13 AB - Marine microorganisms are the important resources for natural drug discovery. However, most of genes are usually in silent and could not express under the condition of traditional culture, which limits the discovery of lead compounds. In the present research, marine fungus Schizophyllum sp. YS-08 was selected as the research object, which was from Yellow Sea of China, to activate its silent genes by changing the medium of nutrients and environmental conditions. The crude extracts from metabolites of marine fungi were analyzed by HPLC, and their antioxidant activity and acetylcholinesterase (AChE) inhibitory activity were studied by DPPH radical-scavenging and Ellman's method, respectively. The results indicated that metabolic pathway of fungus was regulated effectively in different culture conditions, especially in the normal sea water medium with peptone. The antioxidant activity and AChE inhibitory activity increased significantly in comparison with wild strain and increase of the quantity of chemical constituents were even more. It is also observed that the strains showed relatively slow growth in the high salinity situation, while this adversely environmental condition could promote and produce more active metabolites. Furthermore, 6 mutant strains were obtained under salt stress and were identified. Interestingly, we found that all mutant strains had potency toward antioxidant and AChE inhibitory activity. Among them, YS-08-2 was the most potent, scavenging more than 56.02% towards DPPH free radicals, and YS-08-1, YS-08-4 and YS-08-5 exhibited more than 40% inhibition against AChE. HPLC analysis of extracts showed the metabolites of most fungi were more abundant after regulation of culture conditions. VL - 6 IS - 3 ER -
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